Journal: Cell Transplantation

Article Title: Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

doi: 10.1177/09636897221107009

Figure Lengend Snippet: Antibodies Used for Immunofluorescence Staining.

Article Snippet: Ab16667 , Ki67 , abcam.

Techniques: Immunofluorescence, Staining

Journal: Cell Transplantation

Article Title: Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

doi: 10.1177/09636897221107009

Figure Lengend Snippet: Similar differentiation profile of induced-proliferating NPCs or previously frozen and induced NPCs in vitro . (A, B) Bright field morphology of proliferating and previously frozen-washed and plated NPCs. The image from previously frozen cells was taken at 48 h after plating in NPC media. (C, D) Co-staining with Nestin and MAP2 antibodies shows a mixed population of Nestin+, MAP2+, or Nestin/MAP2 expressing cells. (E, F) Presence of early postmitotic DCX+ neurons and early glial VIM + precursors. (G, H) Staining with the Tuj1 antibody show well-developed neurons with axodendritic arborization. In the same field, a high density of mitotically active cells (Ki67+) is also seen. (I) Quantitative analysis of Nestin, MAP2, DCX, Tuj1, and Ki67-positive cells showed no significant difference between freshly induced and frozen-washed and induced NPCs. Scale bars: 200 µm (A–H). NPC: neural precursors cell; VIM: Vimentin; DAPI: 4′,6-diamidino-2-phenylindole.

Article Snippet: Ab16667 , Ki67 , abcam.

Techniques: In Vitro, Staining, Expressing

Journal: Cell Transplantation

Article Title: Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

doi: 10.1177/09636897221107009

Figure Lengend Snippet: Comparable survival and differentiation of freshly harvested or previously frozen-washed NPCs grafted into striata of the immunodeficient rat. (A, B) Comparable engraftment of hNUMA + cells with DCX and hGFAP immunoreactivity in the core of the graft. (C, D) Expression of NeuN in hNUMA+ neurons. Homogenous vimentin IF in the same areas can also be seen. (E, F) Sparse presence of grafted cells-derived oligodendrocytes (Olig2). (G, H) Co-expression of Ki67 mitotic marker in grafted hNUMA cells. (I) Quantitative analysis of neuronal, glial, and mitotic marker expression in grafted cells. No significant differences in differentiation profile between NPCs grafted as freshly harvested or previously frozen cells can be seen. Scale bars: 20 µm (A-H), 10 µm (A-H inserts). NPC: neural precursors cell; VIM: Vimentin; DAPI: 4′,6-diamidino-2-phenylindole; hGFAP-human-specific glial fibrillary acidic protein.

Article Snippet: Ab16667 , Ki67 , abcam.

Techniques: Expressing, Derivative Assay, Marker

Journal: Cell Transplantation

Article Title: Expandable Sendai-Virus-Reprogrammed Human iPSC-Neuronal Precursors: In Vivo Post-Grafting Safety Characterization in Rats and Adult Pig

doi: 10.1177/09636897221107009

Figure Lengend Snippet: Survival and differentiation of freshly harvested or previously frozen-washed NPCs grafted into the lumbar spinal cord of the immunodeficient rat. (A, B) Presence of hNUMA+ cells with dense DCX immunoreactivity in early postmitotic neurons. hGFAP immunoreactivity throughout the grafts can also be seen. (C, D) NeuN expression in mature neurons in the core of hNUMA+ grafts. Vimentin positivity was typically seen at the periphery of the grafts. (E, F) Presence of grafted cells-derived oligodendrocytes (Olig2) throughout the grafts. (G, H) The occasional presence of mitotically active cells (Ki67) in hNUMA + grafts. (I) Quantitative analysis of neuronal, glial, and mitotic marker expression in grafted cells. No significant differences between NPCs grafted as freshly -harvested or previously frozen cells were identified. Scale bars: 20 µm (A-H), 10 µm (A-H inserts). NPC: neural precursors cell; VIM: Vimentin; DAPI: 4′,6-diamidino-2-phenylindole; hGFAP-human-specific glial fibrillary acidic protein.

Article Snippet: Ab16667 , Ki67 , abcam.

Techniques: Expressing, Derivative Assay, Marker

Journal: STAR Protocols

Article Title: Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

doi: 10.1016/j.xpro.2022.101362

Figure Lengend Snippet:

Article Snippet: Anti-Ki-67 (B56)-168Er—100 Tests (1:100) , Fluidigm , Cat#3168007B.

Techniques: Recombinant, Saline, Staining, Blocking Assay, Isolation, Software, Cell Counting, Cytometry

Journal: Journal of Clinical Investigation

Article Title: Loss of acinar cell IKKα triggers spontaneous pancreatitis in mice

doi: 10.1172/jci64498

Figure Lengend Snippet: Figure 2 Loss of pancreatic IKKα causes acinar cell death and compensa- tory proliferation. (A) Amylase and lipase activities in sera of 6- or 12-week-old IkkαF/F and IkkαΔpan mice. (B) Trypsin activity in pan- creata of 2- or 5-month-old IkkαF/F and IkkαΔpan mice. (C) TUNEL assay and apoptotic cell index. Original magnification, ×200. (D and E) H&E staining of IkkαΔpan pancreatic sections showing aci- nar cell apoptosis (D) and necrosis (E). Original magnification, ×400. (F) BrdU incorporation and label- ing index in pancreata of 3-month- old IkkαF/F and IkkαΔpan mice. Original magnification, ×400. (G) Ki67 staining and labeling index of pancreatic sections from 3-month- old mice. Original magnifica- tion, ×400. Results are shown as mean ± SEM. n = 4–6 mice per condition. **P < 0.01. Scale bars: 50 μm; 10 μm (insets).

Article Snippet: IB, IHC, and IF were conducted with antibodies to the following: IKKα (Imgenex), LC3B (Cell Signaling), p62 (Santa Cruz Biotechnology Inc. and Promgen), ERK (Cell Signaling), HA (Roche), Flag (Sigma-Aldrich), ATG16L2 (Abgent), Ub (Santa Cruz Biotechnology Inc.), LAMP1 and LAMP2 (Santa Cruz Biotechnology Inc.), Ki67 (Novocastra Laboratories), B220 (BD Biosciences — Pharmingen), CD3 (Abcam), F4/80 (AbD Serotec), PE-Gr1 (BD Biosciences — Pharmingen),insulin (Zymed), glucagon (Abcam), Sp1 (Santa Cruz Biotechnology Inc.), NF-κB/RelA (Santa Cruz Biotechnology Inc.), tubulin (Sigma-Aldrich), phospho-elF2α (Upstate), elF2α (Santa Cruz Biotechnology Inc.), phospho-ERK (Cell Signaling), JNK1/2 (Pharmingen), phospho-JNK (Cell Signaling), cathepsin B and cathepsin D (Santa Cruz Biotechnology Inc.), phospho-PERK (Cell Signaling), phospho-IKKα/β (Cell Signaling).

Techniques: Activity Assay, TUNEL Assay, Staining, BrdU Incorporation Assay, Labeling

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood

doi: 10.1007/978-1-0716-2553-8_10

Figure Lengend Snippet: Whole blood phosphoflow panel 1

Article Snippet: 172 Yb , Anti-Ki-67 , B56 , Fluidigm 3172024B.

Techniques:

Journal: Frontiers in Pediatrics

Article Title: Neurogenesis Is Reduced at 48 h in the Subventricular Zone Independent of Cell Death in a Piglet Model of Perinatal Hypoxia-Ischemia

doi: 10.3389/fped.2022.793189

Figure Lengend Snippet: Fluorescent microphotographs of Ki67 [cell proliferation, (A,B) ], Sox2/Ki67 [neural stem/progenitor cell proliferation, (C,D) ], and DAPI [nuclei, (E,F) ] immune-stained samples from control and HI piglets. Ki67 positive cells (green) are abundant in the control piglet (A) , whereas HI (B) significantly reduced its counts by half in L-SVZ and DL-SVZ (both *** p < 0.001). Double Sox2 (red) + /Ki67 (green) + cells are observed in control animals; again, HI reduced neural stem/progenitor cell counts in L-SVZ and DL-SVZ (both *** p < 0.001). DAPI immunostaining reveals total nuclei. LV, lateral ventricle; SVZ, subventricular zone; CDT, caudate nucleus; PvWM, periventricular white matter. Original magnification 400×. Scale bar: 100 μm.

Article Snippet: For single labeling, radial-glia/neural stem cells were identified using an anti-glial fibrillary acidic protein (GFAP) antibody to assess neurogenic activity close to the ventricular wall (mouse anti-GFAP, 1:100, MA5-12023, Thermo Fisher, United States); an anti-doublecortin (DCX) antibody was used to identify young neurons/neuroblasts (mouse anti-DCX, 1:50, sc-271390, Santa Cruz Biotechnology, United States); cell proliferation was identified by an anti-Ki67 antibody (mouse anti-Ki67; 1:50, STJ96966, St Johns Labs, United Kingdom).

Techniques: Staining, Control, Immunostaining

Journal: Cancer cell

Article Title: Dendritic cell paucity leads to dysfunctional immune surveillance in pancreatic cancer

doi: 10.1016/j.ccell.2020.02.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: anti-human Ki-67 (B56) , Fluidigm , #3161007B.

Techniques: Recombinant, Cell Stimulation, Enzyme-linked Immunosorbent Assay, CyQUANT Assay, LDH Cytotoxicity Assay, Expressing, Staining, Derivative Assay, Software

Journal: Cell reports

Article Title: Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry

doi: 10.1016/j.celrep.2021.109919

Figure Lengend Snippet:

Article Snippet: 168Er-Ki67 (Clone B56) , Fluidigm , Cat#3168022D.

Techniques: Software

Journal: STAR Protocols

Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

doi: 10.1016/j.xpro.2022.101949

Figure Lengend Snippet:

Article Snippet: Anti-Mouse KI67-162Dy dilution 1:200 , Standard BioTools , 3162012B.

Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy